Microbiology
✅Jawetz Melnick & Adelbergs Medical microbiology
Considered as a single best resource for microbiology and immunology by students, teachers and health care professionals all around the world. Fundamental concepts of organisms and their role in disease, diagnosis, treatment and epidemiology are explained. Case studies with differential diagnosis and algorithm of lad diagnosis are provided. All the latest techniques and updates are done in newer editions.
✅Ananthnarayan & Paniker’s Microbiology
One of the trusted books in microbiology that has concepts integrated with a system-wise approach. Essential concepts are given more preference and various flowcharts, tables, and pictures make students oriented. National programs related to respective topics are added with prevention and control protocols. Concepts are explained in a systematic way.
✅Apurba Sastry – Essentials of Microbiology
A comprehensive and precise explanation of topics in microbiology. Explained in a point-wise manner that makes the information retainable and reproducible in exams. Numerous images, flow charts, and tables make understanding easy for students. Detailed explanations of all the important topics are explained which help students in preparation for entrance exams.
✅Lippincott’s Microbiology
Emphasis on clinically important microbiological concepts with a summary of important bacteria. Various case studies are explained for integration and to apply the concept in real case scenarios. This book focuses more on clinical aspects and ensures students learn the basic concept of diagnosis and investigation. Self-assessment questions and other formats of questions are provided in each chapter to aid in quick revision.
✅Medical Parasitology – Apurba Sastry
More content is precisely explained in a point-wise manner with flowcharts and illustrations. Real images and schematic diagrams of the life cycle make understanding easy. Explained in simple and lucid language. The summary of each chapter is added for quick revision. Multiple choice questions are given to make students oriented to the entrance pattern examination.
✅Paniker’s textbook of Parasitology
Comprehensive parasitology book by Paniker written in a systematic format to make students interested and to get the proper orientation of the subject. Different clinical aspects, diagnosis and prophylaxis protocols are explained well. Explained in a point-wise manner which makes concepts remembering easy. New photographs and tables are included in the new edition.
So what is
microbiology? Microbiology: is the study
of microscopic organisms such as:
bacteria, viruses, fungi, and protozoa. most of these microorganisms are beneficial
to us. In humans we have normal
microbiota that helps in digestion. In
plants the bacteria is responsible for
nitrogen fixation and in animals it
helps in cellulose digestion. Also these
microorganisms are used in industries.
Generally we divide the organisms these
bacteria viruses and fungi according to
their characteristics.
So in bacteria we use Gram stain either:
positive or negative to categorize them.
In viruses we depend on their genome:
DNA or RNA, whether it's single stranded or double stranded to categorize them.
In fungi they're either divided into: molds
which is multicellular, or yeasts which are unicellular.
We also have the hierarchy of classification we used to
put each organism including us humans
into its category "we took it in high
school if you remember". So from highest
to lowest it's: domain > kingdom > phylum >
class > order > family > genus > and species. You
can use this mnemonic to help you
remember
Dina's kids prefer candy over fried
green spinach. Spinach is pretty
underrated by the way 00:01:48,570
from the top we have three domains on
this planet bacteria, archaea and Eukarya.
Both bacteria and archaea are considered
as prokaryotes whereas Eukarya are
considered as eukaryotes. The main
differences between prokaryotes and
eukaryotes are absence of cytoplasmic
structures such as mitochondria Golgi
apparatus, and endoplasmic reticulum in
prokaryotes, and then we have absence of
cell wall in eukaryotes except for fungi
and plants also they differ in the
structure of the ribosomes.
After that we go to the kingdom both
bacteria and archaea have only one
single kingdom which are bacteria and
archaea, respectively. And then we have
the eukarya and it has four kingdoms:
protists, fungi, plants, and animals. And
then we continue the classification like
this like we mentioned before phylum
class order family genus and then species.
For now we will focus on bacteria how do
we name them basic structures in gram
staining. Any bacteria's name is composed
of two words: the first one refers to the
name of the genus, and the second one
refers to the species name. We'll take
Staphylococcus aureus as an example. So Staphylococcus is the genus and aureus
is species. Also the name of the
microorganism might refer to its shape.
Like an our example staphylo means a
bunch of grapes or a cluster and coccus
means round cells aureus means it looks
like gold in color when we culture. Other
than cocci the round cells we have: rods
or bacilli, and then we have spirochete:
which are curved, and then we have diplococci which is basically two cocci or
basically two round cells, and then we
have cocco-bacilli which is a mixture of
both a rod and a round cell, and then we
have vibrios which is a curved rod, then
we have streptococci
which are cocci in chains, and then we
have the staphylococci like we mentioned
in our example which are large cocci in
irregular clusters, and then we have
tetrads which are cocci in a packet of
four from the name tetrads tetra which
means four. Now we're going to discuss
the bacterial architecture. Since
bacteria are considered as cells that
means they have: genetic material, a cell
membrane, and a cytoplasm. In addition to
that they have a cell wall, a flagella
which is used for locomotion, and then we
have pili which is used for
attachment, and then we have plasmid
which is an extra chromosome of genetic
material so it's not part of the DNA, and
then we have a glycocalyx which is used
for protection. The glycocalyx can be
either capsule which is neatly organized
and protects the bacterium from
phagocytes, or biofilm which is an
unorganized loose
structure yet important for attachment.
But here also have porins these porins
are transmembrane proteins that
selectively allow some molecules to pass
the cytosol of the bacteria. Porins can
mediate antibiotic resistance by
inhibiting some antibiotics from
entering the bacteria. They also have
Mesosomes that are equivalent to mitochondria in eukaryotes. Remember bacteria
doesn't have mitochondria so they have
Mesosomes instead bacteria depend on
these Mesosomes for respiration. Mesosomes are formed by the invaginations of
a plasma membrane into the cytosol.
Generally speaking this is how a bacterium
looks like; however, each bacterium has
its special characteristics that
distinguish it from the rest. Now move on
to gram staining. In gram staining we can
classify bacteria into either gram
positive or gram negative and that
depends on the cell wall constituents
and its ability to react with the Gram
stain. To classify them gram positive
react with the stain and give blue color,
while gram negative react with the stain
and give red or pink color. If we look at
the membranes of both gram-positive and
gram-negative bacteria, from the inner
layer, we see that both of them have
plasma membrane; however, on the outside
the differences start appear. In gram
positive it has a thick peptidoglycan
layer. This layer has two techoic and
lipotechoic acids. In gram negative
bacteria and outer to the plasma
membrane we have: thin layer of
peptidoglycan, and then we have outer
membrane composed of phospholipids that
has trans-proteins and lipoproteins
embedded into it and the last layer in
gram-negative bacteria is a polysaccharide. The lipopolysaccharide
layer is composed of three structures: o-antigen, core polysaccharide, lipid A,
which is also known as endotoxin. So we
can conclude that the endotoxins are
only present in gram-negative bacteria.
These endotoxins are antigenic which
means they can elicit an immune response
causing some symptoms of: fever, weakness,
aches, or shock to the patient.
So that's it for the intro hope you enjoyed!
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